Fig. 8. UPLC traces of crude reaction mixtures obtained after aqueous work-up in conversion of the pre-ZIP intermediate to the templated product: (a) the starting pre-ZIP intermediate and the crude products obtained after (b) the CuAAC ZIP step, (c) LiOH base-pair cleavage, (d) CuAAC capping of the copy strand. Conditions: C18 column at 40 °C using a gradient of water/formic acid (0.1%) and CH₃CN/formic acid (0.1%). The capping azide appears at different retention times in (b) and (c), because the gradient used in (a) and (b) was different from that used in (c) and (d).