Figure 1.

KML001 inhibits CD4 T cell proliferation, cytokine production, and induces apoptotic death. Healthy PBMCs were cultured in the presence or absence of TCR stimulation and varying concentrations of KML001 for different times, followed by measuring T cell proliferation, cytokine production, and apoptosis by flow cytometry. (A) KML001 inhibits CD4 T cell proliferation in a dose-dependent manner, measured by CFSE dilution in dividing cells. (B,C) KML001 inhibits IL-2 and IFN-γ productions in TCR-stimulated CD4 T cells. Representative dot plots and summary data from 8 subjects per group are shown. (D) KML001 promotes CD4 T apoptotic death, in a dose- and time-dependent manner, determined by the percentage of Av/7AAD positive cells.