Table 1.
Demographics and Candidate Biomarker Values for SMART-AV Patients and Referent Normal Subjects*
| Characteristic | Statistic | Referent Normal (N = 26) |
Smart-AV (N =921) |
p-value | Responders (N =391) |
Non-Responders (N = 367) |
p-value |
|---|---|---|---|---|---|---|---|
| Age (y) | Mean ± SE Median (25th, 75th %ile) |
66.6 ± 1.8 66.0 (63.0, 73.0) |
66.2 ± 0.4 67.0 (59.0, 75.0) |
0.97 | 65.9 ± 0.5 66.0 (59.0, 74.0) |
65.7 ± 0.6 67.0 (58.0, 74.0) |
0.91 |
| Gender | M/F (% M) | 18/8 (69.2%) | 625/296 (67.9%) | 0.88 | 244/147 (62.4%) | 263/104 (71.7%) | 0.007 |
| LBBB | N (%) | Not Collected | 692 (75%) | N/A | 322 (82%) | 252 (69%) | < 0.001 |
| LVEF (%) | Mean ± SE Median (25th, 75th %ile) |
67.9 ± 1.4 67.0 (63.8, 72.6) |
27.5 ± 0.3 26.9 (21.0, 33.9) |
< 0.001 | 25.7 ± 0.4 25.3 (20.0, 31.0) |
30.0 ± 0.5 29.8 (23.1, 36.8) |
< 0.001 |
| LVESV (mL) | Mean ± SE Median (25th, 75th %ile) |
35 ± 2 34 (26, 43) |
130 ± 2 117 (84, 157) |
< 0.001 | 144 ± 3 128 (98, 170) |
117 ± 3 103 (75, 146) |
< 0.001 |
| CRP (ng/mL) | Mean ± SE Median (25th, 75th %ile) |
2984 ± 617 1779 (1067, 3400) |
6514 ± 146 5436 (2831, 9756) |
< 0.001 | 5874 ± 211 4784 (2383, 8199) |
6806 ± 237 5632 (2941, 10646) |
0.004 |
| sGp130 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
165123 ± 8951 162625 (123059, 207369) |
200198 ± 2144 196123 (154450, 244425) |
0.006 | 196763 ± 32 6 191736 (150888, 235030) |
200837 ± 3342 196943 (157929, 246126) |
0.27 |
| sIL-2ra (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
846 ± 70 762 (598, 974) |
1145 ± 21 1015 (701, 1410) |
0.008 | 1134 ± 34 978 (695, 1375) |
1141 ± 33 1027 (697, 1418) |
0.68 |
| sTNFr-II (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
6404 ± 621 5259 (4672, 6542) |
9250 ± 263 7635 (5333, 10929) |
0.002 | 8913 ± 493 6950 (5260, 9939) |
9190 ± 286 8052 (5396, 11322) |
0.003 |
| IFNg (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
3.2 ± 0.8 2.4 (2.4, 2.6) |
4.3 ± 0.5 2.9 (2.6, 3.2) |
< 0.001 | 3.4 ± 0.2 2.9 (2.7, 3.2) |
5.5 ± 1.3 2.8 (2.6, 3.3) |
0.56 |
| NT-proBNP (pmol/L) | Mean ± SE Median (25th, 75th %ile) |
851 ± 104 673 (467, 1294) |
2952 ± 96 1735 (874, 4045) |
< 0.001 | 2631 ± 139 1534 (820, 3601) |
2877 ± 145 1679 (846, 3897) |
0.16 |
| sST-2 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
23741 ± 2094 22056 (18079, 26244) |
34720 ± 762 28557 (20462, 41837) |
0.003 | 31184 ± 987 26769 (18394, 37317) |
35749 ± 1197 29748 (21583, 42503) |
0.001 |
| MMP-2 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
790795 ± 45595 770113 (666576, 905448) |
877129 ± 15968 767645 (536830, 1104317) |
0.98 | 832740 ± 22341 724524 (519381, 1010567) |
899773 ± 27573 767645 (534363, 1135681) |
0.13 |
| MMP-9 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
191221 ± 19994 186979 (91462, 295038) |
146066 ± 4779 106801 (67204, 172259) |
0.004 | 144978 ± 6879 106801 (69433, 167351) |
149221 ± 8196 108319 (70022, 179013) |
0.80 |
| TIMP-1 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
142776 ± 6463 138676 (124832, 167069) |
139950 ± 2303 122630 (90070, 176917) |
0.12 | 136534 ± 3572 118659 (90029, 169334) |
138547 ± 3429 122867 (88969, 184127) |
0.44 |
| TIMP-2 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
104209 ± 2799 106349 (95839, 110460) |
105103 ±9 25 101823 (86769, 120942) |
0.68 | 103811 ± 1338 101243 (86066, 119650) |
106265 ± 1585 101533 (86468, 126132) |
0.34 |
| TIMP-4 (pg/mL) | Mean ± SE Median (25th, 75th %ile) |
2510 ± 207 2112 (1905, 3087) |
2608 ± 52 2263 (1550, 3225) |
0.77 | 2618 ± 85 2249 (1524, 3301) |
2599 ± 81 2276 (1575, 3201) |
0.65 |
*
The 12 candidate biomarkers shown here were selected from an initial screening of 74 biomarkers, and these 12 were then subjected to further analysis for developing a final composite Biomarker CRT Score. The clinical demographic variables were considered as covariates/confounding variables in developing predictive models for the Biomarker CRT Score. Referent Normal values are presented as a frame of reference for the SMART-AV patient values.