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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jun;39(6):1212–1226. doi: 10.1161/ATVBAHA.119.312729

Figure 5. Gαq/11 mediate thrombin-induced IL-33 expression and HASMC migration.

Figure 5.

A. HASMCs were transfected with the indicated siRNA (100 nmoles), quiesced, treated with and without thrombin (0.5 U/ml) for 30 min and cell extracts were prepared and analyzed for PLCβ3 activity. B, C, E & F. All the conditions were the same as in panel A except that cells were treated with and without thrombin (0.5 U/ml) for 6 hrs and either RNA was isolated or cell extracts were prepared. The RNA and cell extracts were analyzed by RT-PCR and Western blotting, respectively, for IL-33 and β-actin mRNA and protein levels using their specific primers or antibodies, respectively. Cell extracts were also analyzed by Western blotting for Gαq and Gα11 levels to show the efficacy of their respective siRNA. D & G. HASMCs were transfected with the indicated siRNA, quiesced and subjected to migration assay. The bar graphs represent the Mean ± S.D. values of three independent experiments. *, p < 0.05 versus siControl; **, p < 0.05 versus siControl + thrombin.