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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jun;39(6):1212–1226. doi: 10.1161/ATVBAHA.119.312729

Figure 7. IL-33 mediates injury-induced neointima formation.

Figure 7.

A. Tissue extracts prepared from uninjured and 3 days of post guide wire-injured mouse femoral arteries were analyzed by Western blotting for IL-33 levels using its specific antibodies and normalized to α-tubulin levels. B. An equal amount of protein from 3 days of post guide wire-injured arteries that received either siControl, siNFATc1 or siE2F1 (10 μg/artery) or uninjured arteries were analyzed by Western blotting for IL-33 using its specific antibody and the blot was reprobed for NFATc1, E2F1 or α-tubulin to show the efficacy of the siRNA on its target and off target molecule levels. C. Uninjured and 3 wks of post guide wire-injured mouse femoral artery cross sections were co-immunostained for IL-33 (red) and SMC α-actin (green). The sections of injured artery were also stained for control mouse and goat IgG. D. Mice were administered with normal IgG or neutralizing IL-33 antibodies and subjected to GI. Femoral arteries from uninjured and 3 wks of post-GI mice were dissected out, fixed, cross sections were made and stained with H&E. The I/M ratios were calculated. Representative pictures of GI femoral artery cross-sections that were stained with H&E are shown and bar graph shows Mean ± S.D. values of the I/M ratios of the femoral arteries from injured and injured + IL-33 nAb administered mice (n = 6). E. Left panel: Femoral arteries from 5 days of post-GI mice that were administered with IL-33 nAb or normal IgG (2 μg/mouse by IP) were isolated, opened longitudinally and stained for SMCα-actin. Right panel: the bar graph shows Mean ± S.D. values of the SMCα-actin-positive cells from injured and injured + IL-33 nAb administered mice (n = 6). F. Paraffin-embedded human normal and atherosclerotic artery sections were co-immunostained for IL-33 (red) and SMCα-actin (green). The normal human artery sections were also stained for control mouse and rabbit IgG. Scale bars are 50 μm (panels C, D & F) and 20 μm (panel E). *, p < 0.05 versus injured + nIL-33 Ab.