Figure 7.

Comparison of EA.hy926 and primary human umbilical vein endothelial cell (HUVEC) cell attachment on PVA surfaces without plasma treatment and with NH₃ plasma treatment. (A) Fluorescence images of EA.hy926 cells fixed on day 30 of culture and of (B) HUVECs fixed on day 16 of culture on unpatterned and patterned PVA. Nuclei and F-actin were stained blue and green, respectively. Double-headed arrows indicate gratings direction. (C) The stability of cell adhesion on the PVA surfaces with and without plasma treatment was further studied and compared on unpatterned sample up to 30 days of culture. Cells were almost confluent on day 14 on plasma treated surfaces and maintained their attachment as observed on day 30. Scale bar: 50 μm. (D) Cell number quantification of EA.hy926 cells (day 30, N = 1 for each groups), HUVECs (day 16, N = 2 for each groups), and stability study with EA. hy926 cells (day 2, 9, and 14, N = 1 and 2 for without plasma and NH₃ plasma groups, respectively; day 12, N = 2 and 3 for without plasma and NH₃ plasma groups, respectively; and day 30, N = 1 for each groups).