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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cancer Res. 2018 Nov 1;79(1):231–241. doi: 10.1158/0008-5472.CAN-18-1089

Figure 2. Effects of FLT4 rs307826 (A>G, T494A) on VEGFR-3 phosphorylation and post-translational processing.

Figure 2

A) VEGFR-3 phosphorylation with and without VEGF-C stimulation in HUVECs transfected with either the A (WT) or the G (T494A) allele. Notably, the major precursor of VEGFR-3 (170 kDa) matures to a 190 kDa fully glycosylated cell-surface VEGFR-3, which is then proteolytically cleaved into 125 kDa C-terminal fragment, and a shorter N-terminal fragment (not visible). Please note that pY indicates phosphorylated VEGFR-3 forms (top gel). B) Quantification of the 125:170 kDa VEGFR-3 ratio bands in the experiments reported in the A panel. The mean±SEM is shown. ** P≤ 0.01. C) Pulse-chase analysis of metabolically-labeled and streptactin sepharose-precipitated VEGFR-3 polypeptides.