a.
Selenbpl has multiple proximal Nkx2-1 ChlP-seq peaks and SELENBP1 expression correlates with Nkx2-1 expression in human lung adenocarcinoma. Each dot represents a ChlP-seq peak associated with a particular gene.
b, c.
SELENBP1 expression correlates with Nkx2-1 expression (b), and the expression of the Nkx2-1 target gene surfactant protein b (SFTPB)(c) in human lung adenocarcinoma. Each dot represents a tumor.
d.
SELENBP1 expression correlates with SFTPB expression in human lung adenocarcinoma. Each dot represents a tumor.
e. Representative immunohistochemical staining of an Nkx2-1positive and an Nkx2-1negativea, human lung adenocarcinoma. Scale bar = 100 μm.
f. SELENBP1 and Nkx2-1 protein levels scored as low/negative (low/neg) or positive (pos) from human lung adenocarcinoma TMA. The relationship between SELENBP1 and Nkx2-1 is statistically significant (calculated using a 2×2 contingency table). * P ≤ 0.05.
g. Lung adenocarcinoma patient outcome stratified by SELENBP1 expression. Analysis of 720 patients from GEO (Affymetrix microarrays), EGA, and TCGA (Szasz etal., 2016). P-value and hazard ratio are indicated.
h. ChlP-seq data for Nkx2-1, Foxa1/Foxa2, and histone marks around Selenbp1 in murine lung tumors.