Table 1. Primer sequence used for quantitative real-time PCR.
| Gene of interest | GenBank accession | Position | Sequence (5’-3’) |
|---|---|---|---|
| 18S | NR_036642.1 | Forward | ACCCATTCGAACGTCTGCCCTATT |
| Reverse | TCCTTGGATGTGGTAGCCGTTTCT | ||
| AMBP | NM_173989.3 | Forward | ACTGTCAAGCTCTATGGGCG |
| Reverse | CCTCTGTCGGGCATTGTGAA | ||
| HMBS | NM_001046207.1 | Forward | GATGGGCAACTGTACCTGACT |
| Reverse | TGGTTTGCATGGTGTCTTGC | ||
| PC | NM_177946.4 | Forward | CCACGAGTTCTCCAACACCT |
| Reverse | TTCTCCTCCAGCTCCTCGTA | ||
| PEPCKc | NM_174737.2 | Forward | AACCTGGCCATGATGAACCCTACT |
| Reverse | ACTCCTTGCCCTTCCAGGAAATGA | ||
| PEPCKm | NM_001205594.1 | Forward | TGACTGGGCAAGGGGAGCCG |
| Reverse | GGGGCCACCCCAAAGAAGCC | ||
| G6PC | NM_001076124.2 | Forward | TGATGGACCAAGAAAGATCCAGGC |
| Reverse | TATGGATTGACCTCACTGGCCCTCTT |