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The first step represents the forward-scatter (FSC) versus side-scatter (SSC) plot (left). In this step, the unassigned events and artifacts (including cell debris, mechanical noise, and cell clumps) are gated off. In the second step, the dead cells and CD235a-positive cells are gated off on the CD235a versus 7-AAD plot (middle). In the remaining CD45-negative cells, GPC3-positive cells are selected with the CD45 versus GPC3 plot (right).
