Table 1. Primer pairs used to construct sgRNA expression vectors.
Primer | Sequence |
---|---|
pCMAH exon 2F | CGTC GAAGCTGCCAATCTCAAGGA GTTTTAGAGCTAGAAAT |
pCMAH exon 2R | TGCTATTTCTAGCTCTAAAAC TCCTTGAGATTGGCAGCTTC |
pCMAH intron 2F | CGTC GATCGCCAGGGAGAAAGCAA GTTTTAGAGCTAGAAAT |
pCMAH intron 2R | TGCTATTTCTAGCTCTAAAAC TTGCTTTCTCCCTGGCGATC |