Table 3. Germline transmission and genotypes of F1 CMAH KO piglets.
| Sow | Parity | Litter size | m/f/d(n)a | Birth weight (Mean±SE), kg |
No. of piglets of KO genotypeb | ||
|---|---|---|---|---|---|---|---|
| D/D (-161/+1/-5) | D/M (site I) | ||||||
| L667-10 | (-14 bp) | ||||||
| 1 | 12 | 5/5/2 (3) | 1.43 ± 0.16 | 6 | (5/1/0) | 6 | |
| 2 | 8 | 5/3/0 (0) | 1.76 ± 0.08 | 7 | (7/0/0) | 1 | |
| 3 | 12 | 3/6/3 (0) | 1.53± 0.06 | 9 | (9/0/0) | 3 | |
| L667-11 | (+1 bp) | ||||||
| 1 | 6 | 4/1/1 (0) | 1.77 ± 0.05 | 4 | (4/0/0) | 2 | |
| 2 | 6 | 4/2/0 (0) | 1.76 ± 0.10 | 3 | (3/0/0) | 3 | |
| 3 | 1 | 0/0/1 (0) | 1.72 | 1 | (1/0/0) | 0 | |
| L667-12 | (-2 bp) | ||||||
| 1 | 13 | 7/3/3 (3) | 1.46 ± 0.10 | 13 | (7/5/1) | 0 | |
| 2 | 13 | 8/4/1 (0) | 1.57 ± 0.08 | 11 | (6/3/2) | 2 | |
| 3 | 12 | 6/3/3 (0) | 1.62 ± 0.07 | 10 | (5/3/2) | 2 | |
| Sum | 58 | 33/18/7 (6) | 1.58 ± 0.04 | 17 | 11/5 | 8 | |
a. m/f/d (n): No. of males/females/stillbirths (n = live piglets that died due to weakness).
b. Genotypes: D indicates deleted and M refers a site I mutation in exon 2 and null CMAH expression. D/D type: -161 indicates a genomic type featuring a 161-bp deletion in which the mutation at sites I and II occurs simultaneously on both chromosomes; +1 indicates the indel with a 161-bp deletion and a 1-bp insertion (+1); and -5 indicates the presence of a 161-bp deletion with simultaneous deletion of 5 additional bp (-5 bp). D/M type: -161 bp/ site I mutated; in parentheses, -14 bp indicates a 14-bp deletion, +1 bp indicates a 1-bp insertion, and -2 bp indicates a 2-bp deletion.