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. 2019 May 16;15(5):e1007774. doi: 10.1371/journal.ppat.1007774

Fig 1. The ORF24-RNA pol II interaction is essential for late gene expression and contributes to viral DNA replication.

Fig 1

iSLK cells harboring WT BAC16, the ORF24RAAAG mutant or the ORF24RAAAG mutant rescue (MR) were reactivated with doxycycline and sodium butyrate for 72 hours. (A) Western blot showing expression of an early protein (ORF59) and a late protein (K8.1) in WT, ORF24RAAAG and ORF24RAAAG-MR. Vinculin was used as a loading control. (B) Progeny virion production was measured by supernatant transfer on to 293T cells and quantified by flow cytometry. (C) DNA replication of different mutants and MRs was measured by qPCR and normalized to the respective uninduced sample. The fold induction of all mutants was normalized to the WT to get the relative change in DNA replication compared to WT.