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L chain isotypic inclusion in λ+ B cells in HKIR+/+/Vκ10 mice. Spleen cells from mice of the indicated genotypes were stained with anti-κ and anti-λ Abs (upper panels) and anti-B220, anti-λ and E4 Abs (lower panels), and analyzed by flow cytometry. Major subpopulations of λ+ B cells are gated. All data are representative of multiple experiments using at least three mice per genotype.
