Skip to main content
. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):398–407. doi: 10.4049/jimmunol.182.1.398

FIGURE 8.

FIGURE 8

λ+ B cells from HKIR+/+/Vκ10 mice respond to Ars-KLH immunization in vivo. FACS-purified λ+ and λ− splenic B cells from HKIR+/+/Vκ10 mice were transferred to recipient C57BL/6.CD45.1 mice that were immunized (i.p.) 12 h later with 100 μg of Ars-KLH in alum. Mice were sacrificed on day 6 after transfer and immunization, and splenocytes were stained with Abs specific for the indicated markers and analyzed by three-color flow cytometry. Gated percentages represent peanut agglutinin-positive (PNA+), CD45.2+ GC B cells. The data are representative of two experiments.