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. Author manuscript; available in PMC: 2019 May 29.
Published in final edited form as: J Immunol. 2009 Jan 1;182(1):398–407. doi: 10.4049/jimmunol.182.1.398

FIGURE 9.

FIGURE 9

λ L chain-containing mAbs from HKIR+/+/Vκ10 and HKIR+/+ mice have little nuclear staining activity. Slides coated with fixed Hep-2 cells were stained with mAbs produced by IgM, λ-expressing hybridomas generated from the indicated mice and counterstained with a FITC-labeled anti-IgM Ab. Digital images were captured on a fluorescence microscope. Dilutions of culture supernatants containing equivalent amounts of IgM (determined by ELISA) from each hybridoma were used. Upper panels, × 200 original magnification; lower panels, × 630 original magnification. The staining patterns are representative of all IgM-λ-expressing hybridomas isolated from the indicated strains of mice.