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Polyacylation ability of reagents studied. (A) Median RNA acylation yields for the new acylating reagents with an 18 nt RNA. 6 μM RNA was treated with 0.8 M reagent, 40% DMSO in water for 4 h at room temp. (B) Representative MALDI-TOF mass spectrum of a polyacylated RNA (with 4). MW of Cy5-tRF-3005 = 6184 Da. Red numbers indicate the number of adducts.
