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. Author manuscript; available in PMC: 2020 Apr 18.
Published in final edited form as: Mol Cell. 2019 Mar 6;74(2):284–295.e5. doi: 10.1016/j.molcel.2019.01.044

Figure 2. Ability of a discontinuity to protect downstream sites from cleavage by purified N-RNase E.

Figure 2.

Monophosphorylated (MonoP) or triphosphorylated (TriP) AC3, TR8, or TR11 RNAs that had been synthesized by in vitro transcription and mixed with TRAP were treated for 4 min with equal amounts of purified N-RNase E (20 nM). The reaction products were then analyzed by Northern blotting as in Figure 1. M, boundary marker between the upstream and downstream cleavage sites. See also Figures S2, S4, and S5.