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. Author manuscript; available in PMC: 2020 Apr 18.
Published in final edited form as: Mol Cell. 2019 Mar 6;74(2):284–295.e5. doi: 10.1016/j.molcel.2019.01.044

Figure 3. Effect of ribosome binding on downstream cleavage in E. coli.

Figure 3.

(A) 5′ UTR of reporter mRNAs containing an upstream open reading frame. Arrows, cleavage sites; white rectangles, protein coding regions.

(B) Relative abundance of 5′ UTR cleavage products for SD-low, SD-med, SD-high, SD-ultra, and SD-ultra+AC10 in wild-type cells. SD-ultra+AC10 is identical to SD-ultra except for the presence of a heteropolymeric (AC)10 spacer 4 nt downstream of the uORF. The sum of the intensities of the bands resulting from cleavage upstream of the uORF was divided by the corresponding sum for cleavage downstream. Data from three biological replicates were used to calculate each mean and standard deviation.