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. 2019 May 10;8:e46629. doi: 10.7554/eLife.46629

Figure 9. The loss of Piccolo leads to diminished levels of synaptic Pra1.

(A) Images of Pclowt/wt and Pclogt/gt neurons stained with Synaptophysin and Pra1 antibodies. Pra1 is present at Synaptophysin positive synapses in Pclowt/wt synapses but is reduced in Pclogt/gt synapses. (B) Detail images of areas indicated in (A). (C) Quantitation of (A). Loss of Piccolo decreases Pra1 intensity at synapses compared to Pclowt/wt synapses (Pclowt/wt = 1 ± 0.03, n = 474 synapses; Pclogt/gt = 0.55 ± 0.02, n = 723; three independent experiments). Scale bar represents 10 μm and 5 μm in zoom image, Error bars in bar graph represent 95% confidence intervals. Numbers given represent mean ± SEM, Student`s t –test. **** denotes p<0.0001.

Figure 9—source data 1. This spreadsheet contains the normalized values used to generate the bar plots shown in Figure 9C.
elife-46629-fig9-data1.xlsx (61.3KB, xlsx)
DOI: 10.7554/eLife.46629.027

Figure 9.

Figure 9—figure supplement 1. Pclo-Znf1-mCh is nicely targeted to synapses.

Figure 9—figure supplement 1.

(A) Images depicting co-localization of Pclo-Znf1-mCh with synapses marked by Synaptophysin. (B) Quantification of (A). 83.82 ± 9.475% of Pclo-Znf1-mCh positive puncta co-localize with Synaptophysin. Scale bar represents 10 μm. Error bars in bar graph represent 95% confidence intervals. Numbers given represent mean ± SEM.