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Characterization of expanded CAR T cells.
| Marker | % positive cells (mean ± SD)* |
|---|---|
| CD8 | 97±1.5 |
| KLRG1(MAFA) | 29±1.7 |
| CD152(CTLA-4) | 2.5±1.5 |
| CD69 | 91±1.8 |
| CD25 | 65±9.8 |
| CD11a/CD18(LFA-1) | 94±0.8 |
| IFNγ | 91±3.1 |
| TNFα | 94±2.2 |
Mean values of 4 CAR T cell lines from repeated experiments.
Expanded CAR T cells were stained with antibodies to the indicated markers on day 8 or 9 and analyzed by flow cytometry. Results are expressed as the percentage of viable GFP+ cells.
