Fig. 1. MAGEs are mammalian-specific genes that are restricted to expression in defined cell types of the testis.

(A) Identification of testis-specific transcripts. (B) Many MAGE genes evolved in eutherian mammals. (C and D) Unsupervised hierarchical clustering of human (C) and mouse (D) MAGE genes based on reverse transcription quantitative polymerase chain reaction (RT-QPCR) expression data. Color key indicates relative log₂ expression (0 to 12). (E) Unsupervised hierarchical clustering of MAGE genes during the first wave of spermatogenesis in the mouse testis (P5 to P56) as measured by RT-QPCR. Color key indicates relative expression (0 to 1). (F) Summary of differential expression of MAGE genes during spermatogenesis.