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. 2019 May 29;5(5):eaav4832. doi: 10.1126/sciadv.aav4832

Fig. 3. Male germline of Mage-a KO mice is hypersensitive to genotoxic stress.

Fig. 3

(A) Schematic of allelic series deleting 2, 6, or all 8 mouse Mage-a genes through CRISPR-Cas9 technology. (B to D) Confirmation of Mage-a KO by genotyping (B), immunoblotting of testis lysates (C), and immunostaining of testis (D). WT, wild type. (E to G) Mage-a genes are not required for fertility under basal conditions; testis weights (n ≥ 6), pregnancy rate (n = 6), and litter size (n ≥ 16) are shown. (H to K) Mage-a genes are required to recover (8 weeks) from busulfan (20 mg/kg) genotoxic stress in mice. Testis weights (n = 4) (H), histology (I), and quantification (n > 200 tubules) (J) are shown. Fertility of mice from weeks 8 to 10 after busulfan treatment was determined (n = 4) (K). Data are means ± SEM. P values determined by Student’s t test, **P < 0.01 and ***P <0.001.