Table 2.
List of antibodies
| Antigen | Antibody | Dilution IH (IF) | Company Catalog # |
|---|---|---|---|
| APP/Aβ (6E10) | IgG1 mouse monoclonal to residues 1–16 of Aβ | 1:1000 | BioLegend #803002 |
| Aβ (MOAB2) | IgG2b mouse monoclonal to Aβ | 1:800 | Gift from M. J. LaDu |
| Aβ42 | IgG rabbit polyclonal to Aβ42 | 1:1000 | Millipore #AB5078 |
| Conformational Tau (Alz50) | IgM mouse monoclonal to tau residues 5–15, 312–322 | 1:1000 (1:50) |
Gift from P. Davies |
| Conformational Tau (MC1) | IgG1 mouse monoclonal to tau residues 5–15, 312–322 | 1:1000 | Gift from P. Davies |
| Phosphorylated Tau (pS422) | IgG rabbit polyclonal to tau phospho-Serine 422 | 1:1000 (1:50) |
Invitrogen # 03151 |
| Phosphorylated Tau (AT8) | IgG1 mouse monoclonal to tau phospo-Serine 202 and phospho-Threonine 205 | 1:1000 (1:50) |
Invitrogen # MN1020 |
| Truncated Tau (TauC3) | IgG1 mouse monoclonal to truncated tau at 421/422 residues | 1:1000 (1:50) |
ThermoFisher # AHB0061 |
| Truncated Tau (MN423) | IgG1 mouse monoclonal to truncated tau at glutamic acid 391 | 1:5000 | Gift from L. Binder |
| Choline Acetyltransferase (ChAT) | IgG goat polyclonal to human placental choline acetyltransferase | 1:800 (1:50) |
Millipore #AB144P |
| Tyrosine Hydroxylase (TH) | IgG rabbit polyclonal to tyrosine hydroxylase | 1:1000 | Invitrogen #OPA1–04050 |
| Neurofilament (SMI-34) | IgG1 mouse monoclonal to phosphorylated neurofilament H | 1:1000 | BioLegend #8535503 |
| Sirtuin 6 | IgG rabbit polyclonal to amino acids 330–348 of human Sirt6 | 1:5000 | Sigma #S4197 |
IH, Immunohistochemistry; IF, Immunofluorescence