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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Hepatology. 2019 Mar 13;69(6):2608–2622. doi: 10.1002/hep.30529

Fig. 5. Bruce liver-specific KO mice are more prone to acute liver injury by DEN.

Fig. 5.

(A) Mouse breeding schematic demonstrates the cross of Bruceloxp/loxp mice with Albumin-Cre positive mice for generation of Bruce liver-specific KO (LKO) mice, genotypes confirmed.

(B) Western blot analysis shows absent of BRUCE protein expression in LKO mice.

(C) No detectible phenotypic differences among WT and LKO mouse livers (2-month age).

(D) Significantly increased mRNA transcription levels of inflammation marker the tumor necrosis factor alpha (TNFα) in LKO livers treated with DEN.

(E) Quantification of Ki67 IHC staining showed a significant increase in compensatory proliferation in LKO livers as compared to WT.

(F) Representative photos of Ki67 IHC staining 10 days post DEN treatment.