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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Hepatology. 2019 Mar 22;69(6):2455–2470. doi: 10.1002/hep.30541

FIG. 3.

FIG. 3.

GDNF blocks the phosphorylation of p70S6 kinase in hepatocytes. (A-C) Western blot analysis of phospho-p70S6K (Thr389) and phospho-4E-BP1 (ser69) levels in RALA255–10G rat hepatocytes cultured for 48h in medium supplemented with or without GDNF (200 ng/ml) and palmitate (0.2 mM) and during the last 24h with or without rapamycin (20 nM). Plotted are means + SEM (***, P<0.001; **, P<0.01. n = 3). (D-F) Western blot analysis of phospho-p70S6K (Thr389) and phospho-4E-BP1 (ser69 levels in primary mouse hepatocytes cultured for 48 h in medium supplemented with or without GDNF (200 ng/ml) and palmitate (0.2 mM) and during the last 24 h with or without rapamycin (20 nM). Plotted are means + SEM (***, P<0.001; **, P<0.01. n = 3).