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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Hepatology. 2019 Mar 13;69(6):2518–2532. doi: 10.1002/hep.30528

Figure 5. Combined treatment of polyIC and PD-L1 Ab boosts innate and adaptive immunity in the liver.

Figure 5.

(A) Mice were transfected with Ras/Myc at day 0. polyIC (4 μg/g) or PBS was i.p. injected at day 14, 16, 18, 20 and 22, and PD-L1 Ab (or isotype IgG) was i.p. injected at day 17, 19 and 21. All mice were sacrificed (SAC) at day 24 for analysis. Top: representative immunostaining of CD45 in liver sections. Magnification: x40; Scale bar: 25 μm. Bottom: quantification of CD45+ cell numbers isolated by MACS per mouse, with the WT group indicating mice without any treatment.

(B) Flow cytometry analysis was performed and quantified for the relative ratios of macrophages, NK, DC and MDSC in the total NPCs.

(C) Flow cytometry analysis was performed and quantified for the relative ratios of CD4, CD8 T cells and B cells in the total NPCs, and Treg in the CD4 T cells.

Data are presented as mean ± SD (A: n=3 per group; B, C: n=6 per group). *p<0.05, **p<0.01, for any other group versus control, or as indicated by horizontal line in (B, C).