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. 2019 May 29;9:8010. doi: 10.1038/s41598-019-44515-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of AFB₁-dhd by HPLC-MS. (a) Chromatogram of a microsomal incubation showing the putative AFB₁-dhd peak (t_R = 6.70) and AFB₁ (t_R = 11.38). (b) Protonated monoisotopic masses found in the 6.70 min peak: the mass of 347.3 Da corresponds to the mass of AFB₁-dhd whereas the mass of 329.3 Da (−18 Da) most likely corresponds to the dehydrated form of AFB₁-dhd.