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. 2019 May 29;9:8028. doi: 10.1038/s41598-019-44361-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MRC-5 cells activated by ATC cells-derived CM enhanced their synthetic and secretory functions. Immunoblot analysis of vimentin in MRC-5 cells grown under normal conditions (control) or exposed to CM derived from ATC cells, 8505c and KTC-2, for 72 h (A). The figure shows a representative Western Blot of 3 independent experiments (n = 3) with duplicate samples for each experimental group. Quantification of relative expression of vimentin protein in human fibroblasts after using GAPDH as loading control [(B) full blot is shown in the Supplemental Information, Full Original Blots-IV]. The same blot was stripped and re-blotted using anti-GAPDH antibody for loading control. Data are expressed as mean ± SD. *p < 0.05 and **p < 0.01.