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. 2019 May 29;9:8028. doi: 10.1038/s41598-019-44361-6

Figure 5.

Figure 5

ATC cells-derived CM increased glucose uptake through ROS generation, in MRC-5 cells. Total intracellular levels of ROS in MRC-5 by flow cytometry. Representative histograms are shown of 2 independent experiments (n = 2) with triplicate samples for each experimental group. (A–C) Basal ROS production in MRC-5 cells grown under normal conditions (A) and incubated with KTC-2-derived CM for 24 h in the absence (B) or presence of the ROS scavenger NAC (10 mM) (C). Glucose uptake using the fluorescent glucose analog, 2-NBDG, followed by flow cytometry. Representative histograms (D–F) of 2 independent experiments (n = 2) with triplicate samples for each experimental group, and quantification (G) are shown. Data are expressed as mean ± SD. Basal glucose uptake in MRC-5 cells grown under normal conditions (D) and incubated with 8505c-derived CM for 24 h in the absence (E) or presence of the ROS scavenger NAC (10 mM) (F). Expression levels of LDH-A (H), HIF-1A (I) and IL-6 (J). mRNA levels by RT-qPCR in MRC-5 grown under normal conditions and incubated with 8505c-derived CM for 24 h in the absence or presence of NAC (10 mM). Data are expressed as mean ± SD of 3 independent experiments (n = 3) with triplicate samples for each experimental group. *p < 0.05, **p < 0.005 and ****p < 0.0001. ns, not significant.