Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 29;9:8028. doi: 10.1038/s41598-019-44361-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Treatment with CM from activated fibroblasts increased proliferation and invasion of FTC-133 cells. (A) Schematic representation of CM preparation from MRC-5 cells and treatment of FTC-133 cells. Cell proliferation (B) and in vitro invasion assays (C) were performed in FTC-133 cells incubated with CM derived from activated MRC-5 cells, as described in the Materials and Methods section. Data are expressed as mean ± SD of 3 independent experiments (n = 3) with quadruplicate samples for each experimental group. (D) Immunoblot analysis of vimentin, E-cadherin and GAPDH as a loading control after treatment with CM derived from activated MRC-5 cells. The figure shows representative Western Blots of 3 independent experiments (n = 3) with duplicate samples for each experimental group. (E) Quantification of relative expression of proteins associated with EMT in FTC-133 after using GAPDH as loading control (Full blots are shown in the Supplemental Information, Full Original Blots-VII). Data are expressed as mean ± SD.*p < 0.05 and **p < 0.01.