Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 29;9:8051. doi: 10.1038/s41598-019-44537-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Morphological reorganization of mESCs during the transition to formative pluripotency. (A) Morphology of cells cultured in 2i + LIF or after 48 h of culture in Diff medium. (B) Individual cell trajectories over time for cells within representative colonies maintained in 2i + LIF or in Diff medium. Cell generation numbers are indicated in colors. (C) Blue and red lines show spatial trajectories for representative cells cultured in ground state conditions or in Diff medium. Dashed lines surrounding the trajectories depict their convex hull. (D) Distribution of mean cell speeds for cells grown in 2i + LIF or in Diff medium, calculated for each cell as the total trajectory divided by the CC-L. N = 516 and 623 cells for naive ground state and differentiation, respectively. Data was transformed to log scale for visualization and analyzed for statistical differences with a Mann Whitney U test. (E) Distribution of cell exploration for cells grown in 2i + LIF or in Diff medium, calculated for each cell as the convex hull area divided by the CC-L. Data was transformed to log scale for visualization and analyzed for statistical differences with a Mann Whitney U test. (F) Colony area vs Time for colonies maintained in 2i + LIF or in Diff medium. N = 11 and 12 colonies for 2i + LIF and Diff medium, respectively. Shaded areas show the 95% confidence interval. (G) Colony density vs Time for colonies maintained in 2i + LIF or in Diff medium. N = 11 and 12 colonies for 2i + LIF and Diff medium, respectively. Shaded areas show the 95% confidence interval. (H) CC-L distribution for center and peripheral cells in 2i + LIF or in differentiating conditions.