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. 2019 May 29;9:8047. doi: 10.1038/s41598-019-44390-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Polyphenols identified in the acorn supplement and measurements for the identification of urolithin A in pig bone extracts. (a) Bar graph showing magnitude of the ion count for individual polyphenols identified in the acorn extracts (error bars St. dev. n = 9/group). (b) Average peak areas for plant secondary metabolites identified from acorn extracts (St. dev. n = 9/compound). (c) Chromatographic retention time for Urolithin A in the authentic standard and Sardinian bone 6070116 is 7.42 and 7.43 mins respectively, a difference of 0.6 seconds. (d) The accurate m/z measurement of Urolithin A in the authentic standard and Sardinian bone provided a difference of 0.85 ppm. (e) Comparison of the relative abundance of the M, M + 1 and M + 2 isotopes for Urolithin A in the authentic standard compared to Sardinian bone showed a 99% similarity. (f) Comparison of the product ion spectra from the collision induced dissociation (CID) of urolithin A (precursor m/z 227.03) in the Sardinian extract (top) and product ion spectra from the analysis of the urolithin A standard (bottom).