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. 2019 May 29;9:8001. doi: 10.1038/s41598-019-44393-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Repopulation of kidney scaffold with iPSC-derived ECs delivered by renal artery. (a) Hematoxylin and eosin staining on decellularized kidney scaffold. Scale bar 50 μm; (b) Hematoxylin and eosin staining shows cellular distribution mainly in glomerular structures. Scale bar 50 μm; (c) Immunofluorescence for CD144 showing iPSC-derived ECs in the glomerular capillary. Scale bar 25 μm; (d) ZO-1 staining. Scale bar 20 μm; (e) Ki67 staining. Scale bar 20 μm; (f) Isotype antibody staining used as negative control. Scale bar 50 μm.