Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 May 29;10:2355. doi: 10.1038/s41467-019-10262-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Design and characterization of ConM SOSIP-ferritin nanoparticles. a Left: linear representation of ConM SOSIP.v7-ferritin. The N-terminus from H. pylori ferritin (purple) is linked to the SOSIP.v7 C-terminus by a Gly-Ser-Gly (GSG) linker. Right: model of eight ConM SOSIP.v7 trimers with gp120 subunits (red) and gp41 subunits (pink), displayed on the H. pylori ferritin nanoparticle (purple, PDB: 3BVE) viewed down the two-fold, three-fold, and four-fold axis of the ferritin particle, respectively. The figure was drawn using PyMol. b The two-dimensional class averages of the negative-stain electron microscopic analyses of ConM SOSIP.v7-ferritin nanoparticle. Example images of the micrographs are shown in Supplementary Fig. 5c. c Representative dynamic light scattering graphs showing the size distribution of ConM SOSIP.v7 trimer and SOSIP.v7-ferritin nanoparticle. d Glycan profile of the ConM SOSIP.v7-ferritin nanoparticle as determined by hydrophilic interaction liquid chromatography-ultra performance liquid chromatography. The circle diagram indicates the relative amounts of Man_5–9GlcNac₂ (M5–M9) and complex glycans. e Thermostability (measured by differential scanning calorimetry) of PGT145-purified ConM SOSIP.v7-ferritin nanoparticle expressed in 293F cells. The ConM SOSIP.v7 trimer melting curve (Fig. 1e) is shown in red for comparison. f Binding of broadly neutralizing antibodies (bNAbs) 2G12 and PGT145 and non-neutralizing antibodies (non-NAbs) F105 and 19b to ConM SOSIP.v7 trimer and SOSIP.v7-ferritin (100 nM SOSIP.v7 equivalent each) measured by biolayer interferometry after incubation at different temperatures. Shown are the mean values of the area under the curve (AUC) derived from the binding curves in Supplementary Fig. 6. g Calcium flux (relative fluorescence) in B cells expressing either VRC01, PGT145, PGT121, or PG16 as the B cell receptor, stimulated with ConM SOSIP.v7 trimers or ConM SOSIP.v7-ferritin nanoparticles