Fig. 6.

Immunogenicity of ConM SOSIP.v7-ferritin in macaques. a Macaque immunization schedule. Macaques were immunized at weeks 0, 4, and 20 (black arrows) and the Ab responses were analyzed at weeks −4, 0, 4, 6, 16, 20, and 22 (gray arrows). b Endpoint antibody-binding titers over time against ConM SOSIP.v7 trimer as measured by D7324-capture enzyme-linked immunosorbent assay. Mean titers (n = 6 animals) with SD are shown. Values are from one experiment performed in duplo. c Neutralization titers with over time against ConM virus. Mean titers (n = 6 animals) with SD are shown. d Neutralization titers against ConM in the presence or absence of ConM SOSIP.v7 D368R, ConM SOSIP.v7 D368R with the V1V2 of BG505, or the ConM V3 peptide. Statistical analysis was performed using the Wilcoxon matched-pairs signed-rank test; *p < 0.05. e Neutralization titers (ID₅₀) from macaque sera samples at week 22 against autologous ConM virus (DUMC), heterologous Tier 1 SF162 virus (AMC), and MW965.26 virus (DUMC), and heterologous ConS and TV1.21 virus (DUMC). Neutralization data from individual animals can be found in Supplementary Table 8. f Relative neutralization titers of sera against a panel of ConS mutants. Neutralization data from individual animals can be found in Supplementary Table 9. Statistical analysis was performed by Kruskal–Wallis test and Dunn’s test to correct for multiple testing. **p < 0.01 and ***p < 0.001