Table 1.
Biophysical characterization of stabilized ConM SOSIP trimers
| SOSIP versiona | v4.2 | v5.2 | v7 | v7-ferritin | ||
|---|---|---|---|---|---|---|
| Production | Yield (mg/L)b | 0.9c | 0.9c | 4.9c | 3.5 | |
| Yield (mg/L)d | 0.2e | 0.7e | 1.0e | ND | ||
| Morphology | NS-EMb | Native-like trimers (%) | ND | ND | ~100c | ND |
| Closed native-like trimers (%) | ND | ND | ~45c | ND | ||
| DLSb | Rh (nm) | ND | ND | 6.4c | 13.4 | |
| Pd (%) | ND | ND | 12.5c | 59.9 | ||
| Thermostabilityf | DSCb | Tm (°C) | 65.1c | ND | 67.8c | 72.0 |
| DSCd | Tm (°C) | 65.6e | 68.8e | 66.7e | ND | |
| Glycan composition | HILIC-UPLCb | Man8 (%) | 21c | ND | 12c | 20 |
| Man9 (%) | 23c | ND | 25c | 24 | ||
| Oligomannose (%) | 75c | ND | 68c | 75 | ||
DLS dynamic light scattering, DSC differential scanning calorimetry, HILIC-UPLC hydrophilic interaction liquid chromatography-ultra performance liquid chromatography, ND not determined, NS-EM negative-stain electron microscopy
aA linear representation and an overview of the modifications made to the trimer variants is shown in Figs. 1b and 2a and Supplementary Table 1
bData were derived from 293F cell-expressed and PGT145-purified SOSIP trimers
cData were derived from SOSIP trimers containing a D7324-tag
dData were derived from 293S cell-expressed and 2G12/SEC-purified SOSIP trimers
eData were derived from SOSIP trimers without a tag
fTm values that were obtained with D7324-tagged SOSIP trimers were up to 0.3 °C higher than those without a tag