Table 2.

Generation of SALL1-KO founder foetuses by cytoplasmic injection of Platinum TALENs and CRISPR/Cas9 into porcine zygotes.

Genome-editing tool (conc.)	Recipient no.	Embryos injected*	Embryos transferred	Embryonic stage at transfer	Pregnancy	Foetuses obtained	Foetuses with mutations (%)	Renal hypoplasia** (%)
Platinum TALENs (2 ng/µl)	M230	179	90	2- to 8-cell	+	8	8 (100)	6 (75.0)
	M231	162	93	2- to 8-cell	+	5	5 (100)	3 (60.0)
	Total	341	183		2	13	13 (100)	9 (69.2)
gRNA/Cas9 (5/20 ng/µl)	#1705	242	157	1-cell	+	9	5 (55.6)	1 (20.0)
	#1706	211	62	2- to 8-cell	+	3	2 (66.7)	2 (66.7)
	Total	453	219		2	12	7 (58.3)	3 (42.9)

^*IVM/IVF-derived embryos at the pronuclear stage.

^**Foetuses with homozygous loss-of-function mutations.