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. 2019 May 29;9:8029. doi: 10.1038/s41598-019-43737-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell based assessment of OCS components on viral vector transduction efficiency. (A) Influence of OCS solution. 1000 particles Ad luciferase per Hela cell were mixed with media and increasing percentage of OCS solution and used to infect Hela cells. RLU were determined 24 hours post infection. Data is shown fold change in RLU compared to untransduced Hela cells. (B) Influence of whole blood. Same as 1A except OCS solution was mixed with whole porcine blood. (C) Influence of blood components. Cell based luminescence assay to measure Ad Luciferase transduction efficiency with OCS solution and pig blood components. (D) Influence of OCS circuitry. Viral transduction efficiency over time while on the circuit with a heart. OCS circuit was set up with washed donor pig blood, Ad Luciferase and the donor heart. (RLU = relative light units).