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. 2019 Mar 15;36(5):979–988. doi: 10.1007/s10815-019-01431-y

Table 1.

Primer sequences of VEGF gene Polymorphisms

Polymorphism AS-PCR
primers
Primer sequences (5′–3′) Allele base pair
VEGF -1154 G/A

Common reverse

G-forward

A-forward

5′-CCC CGC TAC CAG CCG ACTT-3′

5′-GCC CGA GCC GCG TGT GGAG-3′

5′-GCC CGA GCC GCG TGT GGAA-3′

252 bp
VEGF -2549 I/D

I-sense

D-anti-sense

5′-GCTGAGAGTGGGGCTGACTAGGTA-3′

5′GTTTCTGACCTGGCTATTTCCAGG-3′

211 bp

229 bp

VEGF -2578 C/A

Common reverse

C-forward

A-forward

5′TGCCCCAGGGAACAAAGT3′

5′TAGGCCAGACCCTGGCAC3′ 5′TAGGCCAGACCCTGGCAA3′

160 bp
VEGF +936 C/T

Common reverse

C-forward

T-forward

5′GGGTGGGTGTGTCTACAGGA3′

5′GGTCGGGTGACCCAGCAC3′ 5′GGTCGGGTGACCCAGCAT3′

220 bp