Table 1.
Polymorphism | AS-PCR primers |
Primer sequences (5′–3′) | Allele base pair |
---|---|---|---|
VEGF -1154 G/A |
Common reverse G-forward A-forward |
5′-CCC CGC TAC CAG CCG ACTT-3′ 5′-GCC CGA GCC GCG TGT GGAG-3′ 5′-GCC CGA GCC GCG TGT GGAA-3′ |
252 bp |
VEGF -2549 I/D |
I-sense D-anti-sense |
5′-GCTGAGAGTGGGGCTGACTAGGTA-3′ 5′GTTTCTGACCTGGCTATTTCCAGG-3′ |
211 bp 229 bp |
VEGF -2578 C/A |
Common reverse C-forward A-forward |
5′TGCCCCAGGGAACAAAGT3′ 5′TAGGCCAGACCCTGGCAC3′ 5′TAGGCCAGACCCTGGCAA3′ |
160 bp |
VEGF +936 C/T |
Common reverse C-forward T-forward |
5′GGGTGGGTGTGTCTACAGGA3′ 5′GGTCGGGTGACCCAGCAC3′ 5′GGTCGGGTGACCCAGCAT3′ |
220 bp |