Fig. 2.

(a) Forward SHG emission attenuation curves (intensity versus depth) for the four tissue types, normalized by peak power per series every $10\mu \mathrm{m}$. Each line represents the $\text{mean}\pm \mathrm{SEM}$ of $n=3$. No significant change is detected in the VP lobes. (b) Best-fit Monte Carlo simulations to the experimental data of the ${\mathrm{AP}}^{+/+}$ and ${\mathrm{AP}}^{-/-}$ based on the primary and secondary filers and guesses to the conversion efficiency. The $R^2$ values between the data and best simulation are 0.86 and 0.69 for the wt and ${\mathrm{Dcn}}^{-/-}$, respectively. (c) Bar graph of the extracted SHG conversion efficiencies for the AP gland, where the ${\mathrm{Dcn}}^{-/-}$ has significantly lower SHG conversion efficiencies compared to wt ($p=0.0046$).