Table 5.
Effect of 30 d supplementation with 0, 1, or 2% EPA- and DHA-enriched diet on concentration of mRNA from the subcutaneous adipose tissue of ewes in late gestation
| Item1 | Treatments2 | P-value3 | ||||
|---|---|---|---|---|---|---|
| 0% | 1% | 2% | SEM | Linear | Quadratic | |
| Pens (ewes) | 8 | 8 | 8 | |||
| LPL | 4,386.4 | 5,228.0 | 2,117.9 | 1,248.89 | 0.23 | 0.20 |
| ATGL | 1,501 | 1,446 | 1,211 | 235.1 | 0.24 | 0.67 |
| HSL | 5,041 | 4,643 | 3,830 | 704 | 0.13 | 0.76 |
| DGAT1 | 1,162.27 | 609.85 | 695.46 | 194.35 | 0.04 | 0.09 |
| DGAT2 | 4,335.3 | 6,850.8 | 4,868.0 | 1,556.58 | 0.81 | 0.25 |
| SCD | 9,795 | 14,409 | 13,150 | 3,583.1 | 0.50 | 0.46 |
| Δ 5 -desaturase | 158.25 | 119.71 | 187.68 | 37.45 | 0.35 | 0.06 |
| Δ 6 -desaturase | 85.29 | 51.45 | 99.52 | 20.49 | 0.56 | 0.07 |
| ELOVL2 | 19.39 | 23.88 | 20.19 | 1.76 | 0.73 | 0.05 |
| ELOVL4 | 42.07 | 46.72 | 98.86 | 34.95 | 0.26 | 0.58 |
| ELOVL5 | 823.9 | 691.1 | 887.8 | 81.5 | 0.57 | 0.06 |
| FABP4 | 137,835 | 171,332 | 126,268 | 15,610 | 0.59 | 0.05 |
| FAS | 11,822 | 11,648 | 11,361 | 3,410 | 0.91 | 0.98 |
| FATP1 | 80.25 | 44.04 | 55.61 | 13.85 | 0.09 | 0.06 |
| PPARα | 177.4 | 148.5 | 187.9 | 16.5 | 0.55 | 0.02 |
| PPARβ/δ | 115.8 | 92.9 | 94.0 | 17.71 | 0.22 | 0.42 |
| PPARγ | 2,106.74 | 2,283.71 | 2,126.17 | 248.12 | 0.95 | 0.52 |
| RXRα | 270.94 | 199.83 | 282.07 | 44.88 | 0.84 | 0.12 |
| Adiponectin | 31,656 | 43,742 | 37,086 | 7,014 | 0.61 | 0.32 |
| Leptin | 138.0 | 219.2 | 75.42 | 36.4 | 0.25 | 0.02 |
| Resistin | 17.328 | 7.274 | 6.849 | 3.450 | 0.05 | 0.26 |
| 5-LOX | 74.99 | 40.26 | 59.04 | 13.91 | 0.44 | 0.13 |
| COX-2 | 8.84 | 9.66 | 10.42 | 1.41 | 0.44 | 0.98 |
Diets containing 1 or 2% of EPA+DHA are more energy dense diets (NEm, 0.54 or 1.07%, respectively) than the diet 0% EPA+DHA.
1Gene expression is a relative value estimated using the mean of beta-actin, beta-2 microglobulin, ciclophilin A, GAPDH, and PGK1. LPL = lipoprotein lipase; ATGL = adipose triglyceride lipase; HSL = hormone sensitive lipase; DGAT1 = diacylglycerol acyltransferase 1; DGAT2 = diacylglycerol acyltransferase 2; SCD = stearoyl-CoA desaturase; ELOVL2 = elongation of very long chain fatty acid 2; ELVL4 = elongation of very long chain fatty acid 4; ELOVL5 = elongation of very long chain fatty acid 5; FABP4 = fatty acid binding protein 4; FAS = fatty acid synthase; FATP1 = fatty acid transport protein 1; RXR = retinoid X receptor; COX-2 = cyclooxygenase 2; 5-LOX = 5-lipoxygenase.
2Treatments on ewes supplemented with 0, 1, or 2% EPA- and DHA-enriched diet (Strata G133, Virtus Nutrition LLC, Corcoran, CA).
3L = linear effect of treatment; Q = quadratic effect of treatment.