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. 2019 Mar 29;81(5):776–779. doi: 10.1292/jvms.18-0575

Table 1. Primers used for RT-PCR.

Gene Accession number F/R Sequence (5′−3′) Tm (°C) Amplicon Size (bp)
CD31 XM_022422841 F AATCCCAAATTCCACGTCAG 63.6 346
R GAATGGAGCACCACAGGTTT 63.9
VEGFR2 NM_001048024 F GACAACCAGACGGACAGTGGTATG 68.6 157
R ACTGGTAGCCACTCGTTTGGTTAG 66.1
COX2 NM_001003354 F GTTCATTCCTGATCCCCAAG 63.2 186
R TTGAAAAGGCGCAGTTTATG 62.6
NPR1 XM_547577 F CGCATTGAGCTGACGCGAAA 71.7 249
R TGAGGTTGCCATGGGAGCAA 71.1