Table 1. Primers used for RT-PCR.
Gene | Accession number | F/R | Sequence (5′−3′) | Tm (°C) | Amplicon Size (bp) |
---|---|---|---|---|---|
CD31 | XM_022422841 | F | AATCCCAAATTCCACGTCAG | 63.6 | 346 |
R | GAATGGAGCACCACAGGTTT | 63.9 | |||
VEGFR2 | NM_001048024 | F | GACAACCAGACGGACAGTGGTATG | 68.6 | 157 |
R | ACTGGTAGCCACTCGTTTGGTTAG | 66.1 | |||
COX2 | NM_001003354 | F | GTTCATTCCTGATCCCCAAG | 63.2 | 186 |
R | TTGAAAAGGCGCAGTTTATG | 62.6 | |||
NPR1 | XM_547577 | F | CGCATTGAGCTGACGCGAAA | 71.7 | 249 |
R | TGAGGTTGCCATGGGAGCAA | 71.1 |