Table 2.

Central composite rotatable design (CCRD)

Run	Medium components					Laccase activity (U/ml)
	pH	Glucose	Yeast extract	KH2PO4	CuSo4	Experimental		Predicted
1	2.75	34	0.65	1.6	0.2496	1.16
2	11	34	0.65	0.4	0.2496	1.25
3	5.5	17	1.3	0.8	0.4993	5.5
4	5.5	17	1.3	0.8	0.4993	5.45
5	2.75	34	0.65	0.4	0.9986	2.14
6	2.75	34	2.6	1.6	0.9986	2.347
7	11	34	0.65	1.6	0.9986	4.55
8	11	8.5	2.6	0.4	0.2496	1.15
9	11	8.5	2.6	1.6	0.9986	4.52
10	2.75	8.5	2.6	1.6	0.2496	1.17
11	2.75	8.5	0.65	1.6	0.9986	2.15
12	2.75	8.5	2.6	0.4	0.9986	2.19
13	5.5	17	1.3	0.8	0.4993	5.49
14	2.75	8.5	0.65	0.4	0.2496	1.53
15	2.75	17	2.6	0.4	0.2496	1.16
16	11	8.5	0.5	1.6	0.2496	1.24
17	11	8.5	2.6	1.6	0.2496	1.4
18	5.5	17	1.3	0.8	0.4993	5.46
19	11	8.5	0.65	0.4	0.9986	4.4
20	11	34	2.6	0.4	0.9986	4.55
21	11	34	0.65	1.6	0.2496	1.35
22	2.75	8.5	0.65	1.6	0.2496	1.35
23	2.75	8.5	2.6	0.4	0.2496	1.3
24	5.5	17	1.3	0.8	0.4993	5.51
25	5.5	17	1.3	0.8	0.4993	5.55
26	11	34	2.6	0.4	0.2496	1.32
27	2.75	34	0.65	0.4	0.2496	1.32
28	11	34	0.65	0.4	0.9986	4.42	2
29	11	8.5	2.6	1.6	0.2496	1.29
30	11	8.5	0.65	1.6	0.9986	4.42
31	2.75	34	0.65	1.6	0.9986	2.25
32	5.5	17	1.3	0.8	0.4993	5.55	3
33	11	34	2.6	1.6	0.9986	4.92
34	5.5	17	1.3	0.8	0.4993	5.55
35	2.75	34	2.6	1.6	0.2496	1.21
36	11	8.5	0.65	0.4	0.2496	1.38
37	2.75	8.5	2.6	1.6	0.9986	2.24
38	2.75	34	2.6	0.4	0.9986	2.25
39	11	8.5	2.6	0.4	0.9986	4.41
40	2.75	8.5	0.65	0.4	0.9986	2.33
41	5.5	17	1.3	0.8	0.4993	7.85
42	5.5	17	0.325	0.8	0.4993	5.29	5
43	5.5	17	1.3	0.8	0.1053	1.03
44	5.5	0.575	1.3	0.8	0.4993	5.53
45	1.375	17	1.3	0.8	0.4993	1.79
46	5.5	17	1.3	0.8	2.3666	4.73
47	9.48	17	1.3	0.8	0.4993	4.53
48	5.5	17	1.3	0.8	0.4993	7.9	1
49	5.5	17	1.3	9.48	0.4993	5.45
50	5.5	17	1.3	0.592	0.4993	5.3
51	5.5	17	5.2	0.8	0.4993	5.37
52	5.5	17	1.3	0.8	0.4993	7.85
53	5.5	68	1.3	0.8	0.4993	7.85

Results represented as mean ± SD of two independent experiments with three replicates each.