Table 4.
Purification steps | Activity (U/ml) | Protein (mg/ml) | Sp. activity (U/mg) | Yield (%) | Purification fold |
---|---|---|---|---|---|
Culture filtrate | 790 | 315 | 2.50 | 100 | 1 |
Amm. Sul. precipitation | 724 | 78.46 | 9.22 | 91.64 | 3.69 |
Dialysis | 643 | 26 | 24.73 | 81.39 | 9.89 |
DEAE cellulose | 415 | 5.6 | 74.10 | 52.53 | 29.64 |