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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Cancer Cell. 2019 Apr 15;35(4):664–676.e7. doi: 10.1016/j.ccell.2019.03.004

KEY RESOURCES TABLE

REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Active (non-Ser33/37/Thr41-phosphorylated) β-catenin Cell Signaling Cat#8814
Total β-catenin Cell Signaling Cat#8480
GAPDH Cell Signaling Cat#2118
Phospho-p70S6K (Thr389) Cell Signaling Cat#9234
Anti-Myc tag Cell Signaling Cat#2272
Phospho-GSK3αβ(Tyr279/216) Thermo Fisher Cat#OPA1–03083
Total GSK3αβ Cell Signaling Cat#5676
anti-ubiquitin antibody (Lys-48 specific, clone Apu2) Millipore Cat#05–1307
Anti-Rabbit IgG (H+L), F(ab’)2 Fragment Alexa Fluor 488 Conjugate Cell Signaling Cat#4412
Phospho-PERK (Thr980) Cell Signaling Cat#3179
Secondary HRP-linked antibody Cell Signaling Cat#7074
Bacterial and Virus Strains
Biological Samples
T-ALL Patient-Derived Xenograft T-ALL 9 This paper N/A
B-ALL Patient-Derived Xenograft CBAB-21952 This paper N/A
Hypodiploid B-ALL Patient-Derived Xenograft 05–577 This paper N/A
Chemicals, Peptides, and Recombinant Proteins
Asparaginase (pegaspargase) Shire Pharmaceuticals Oncaspar
Human Wnt3A R&D systems Cat#5036-WN-010
Trypan blue Invitrogen Cat#T10282
CHIR99021 Selleckchem Cat#S1263
Human interleukin-6 R&D systems Cat#206-IL-010
Human stem cell factor R&D systems Cat#255-SC
Puromycin Thermo Fisher Cat#A1113803
Blasticidin Thermo Fisher Cat#R21001
Neomycin Thermo Fisher Cat#21810031
Polybrene Merck Millipore Cat#TR-1003-G
Lipofectamine 2000 Invitrogen Cat#11668030
Dexamethasone Sigma-Aldrich Cat#D4902
Vincristine Selleckchem Cat#S1241
Doxorubicin Sigma-Aldrich Cat#D1515
6-Mercaptopurine Abcam Cat#ab142389
Rapamycin Selleckchem Cat#S1039
RAD001 Selleckchem Cat#S1120
AZD2014 Selleckchem Cat#S2783
Thapsigargin Sigma-Aldrich Cat#T9033
BRD0703 (Wagner et al., 2018) N/A
BRD3731 (Wagner et al., 2018) N/A
RIPA buffer Merck Millipore Cat#20–188
PhosSTOP phosphatase inhibitor Roche Cat#4906845001
Laemmli sample buffer Bio-Rad Cat#161–0737
b-mercaptoethanol Sigma-Aldrich Cat#M6250
4–12% bis-tris polyacrylamide gel Thermo Fisher Cat#NP0336
PVDF membrane Thermo Fisher Cat# LC2005
Azidohomoalanine (AHA) AlexaFluor488 Thermo Fisher Cat#C10289
TAMRA alkyne Thermo Fisher Cat#T10183
L-Asparagine Sigma-Aldrich Cat#A4159–25G
L-Glutamine Sigma-Aldrich Cat#G8540
GelCode Blue Stain Reagent Thermo Fisher Cat#24590
RPMI-1640 Thermo Fisher Cat#11875119
Critical Commercial Assays
Caspase Glo 3/7 Assay Promega Cat#G8090
DNeasy Blood and Tissue Kit Qiagen Cat#69504
QIAquick PCR purification kit Qiagen Cat#28104
Blood & Cell Culture DNA Maxi Kit Qiagen Cat#51194
Z-VAD-FMK Caspase Inhibitor Promega Cat #G7231
Deposited Data
Proteomics of CCRF-CEM cells treated with asparaginase and/or BRD0705 ProteomeXchange Dataset ID: PXD013061
Experimental Models: Cell Lines
CCRF-CEM cells ATCC Cat#CCL-119
Jurkat cells ATCC Cat#TIB-152
PF382 cells DSMZ Cat#ACC-38
DND41 cells DSMZ Cat# ACC-525
MOLT4 cells ATCC Cat#CRL-1582
OCI-AML2 cells Alex Kentsis lab N/A
SKM-1 cells Alex Kentsis lab N/A
THP-1 cells Alex Kentsis lab N/A
MHH CALL2 cells DSMZ Cat#ACC-341
Normal human CD34+ hematopoietic progenitors Fred Hutchinson Cancer Research Center https://sharedresources.fredhutch.org/products/cd34-cells
Experimental Models: Organisms/Strains
NRG mice (NOD.Cg-Rag1<tm1Mom> Il2rg<tm1Wjl>/SzJ) Jackson labs 007799
Oligonucleotides
See Table S5.
Recombinant DNA
LentiCas9-Blast Addgene.org Cat#52962
LentiGuide-Puro Addgene.org Cat#52963
Gecko v2 guide RNA library Addgene.org Cat#1000000048 and 1000000049
shLuciferase Broad RNAi Consortium Cat#TRCN0000072243
shNKD2 #1 Broad RNAi Consortium Cat#TRCN0000187580
shNKD2 #3 Broad RNAi Consortium Cat#TRCN0000428381
shLGR6 #2 Broad RNAi Consortium Cat#TRCN0000063619
shLGR6 #4 Broad RNAi Consortium Cat#TRCN0000063621
shAPC #1 Broad RNAi Consortium Cat#TRCN0000010296
shAPC #2 Broad RNAi Consortium Cat#TRCN0000010297
shGSK3α #1 Broad RNAi Consortium Cat#TRCN0000010340
shGSK3α #4 Broad RNAi Consortium Cat#TRCN0000038682
shGSK3β #2 Broad RNAi Consortium Cat#TRCN0000039564
shGSK3β #6 Broad RNAi Consortium Cat#TRCN0000010551
psPAX2 Addgene.org Cat#12260
VSV.G Addgene.org Cat#14888
Gag/pol Addgene.org Cat#14887
pXPR_011 Addgene.org Cat#59702
Constitutively active β-catenin (ΔN90) Addgene.org Cat#36985
FBXW7 wild-type Addgene.org Cat#16652
FBXW7 R465C mutant Addgene.org Cat#16653
FBXW7 wild-type (CS-T3614-LX304–01-B) Genecopoeia.com
FBXW7 R465C mutant (EX-OL03574-LX304-B) Genecopoeia.com
7TGC TOPFlash reporter Addgene.org Cat#24304
Hyperactive ΔN-PSMA4 This paper N/A
GSK3α in pWZL expression vector (Banerji et al., 2012) N/A
Software and Algorithms
CrispRVariantsLite v1.1 (Lindsay et al., 2016) Version 1.1
MAGeCK v.0.5.7 (Li et al., 2014) https://sourceforge.net/projects/mageck
CRISPOR (Haeussler et al., 2016) http://crispor.tefor.net/
RStudio v. 1.1.463 http://www.rstudio.org
Other