KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Active (non-Ser33/37/Thr41-phosphorylated) β-catenin | Cell Signaling | Cat#8814 |
| Total β-catenin | Cell Signaling | Cat#8480 |
| GAPDH | Cell Signaling | Cat#2118 |
| Phospho-p70S6K (Thr389) | Cell Signaling | Cat#9234 |
| Anti-Myc tag | Cell Signaling | Cat#2272 |
| Phospho-GSK3αβ(Tyr279/216) | Thermo Fisher | Cat#OPA1–03083 |
| Total GSK3αβ | Cell Signaling | Cat#5676 |
| anti-ubiquitin antibody (Lys-48 specific, clone Apu2) | Millipore | Cat#05–1307 |
| Anti-Rabbit IgG (H+L), F(ab’)2 Fragment Alexa Fluor 488 Conjugate | Cell Signaling | Cat#4412 |
| Phospho-PERK (Thr980) | Cell Signaling | Cat#3179 |
| Secondary HRP-linked antibody | Cell Signaling | Cat#7074 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| T-ALL Patient-Derived Xenograft T-ALL 9 | This paper | N/A |
| B-ALL Patient-Derived Xenograft CBAB-21952 | This paper | N/A |
| Hypodiploid B-ALL Patient-Derived Xenograft 05–577 | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Asparaginase (pegaspargase) | Shire Pharmaceuticals | Oncaspar |
| Human Wnt3A | R&D systems | Cat#5036-WN-010 |
| Trypan blue | Invitrogen | Cat#T10282 |
| CHIR99021 | Selleckchem | Cat#S1263 |
| Human interleukin-6 | R&D systems | Cat#206-IL-010 |
| Human stem cell factor | R&D systems | Cat#255-SC |
| Puromycin | Thermo Fisher | Cat#A1113803 |
| Blasticidin | Thermo Fisher | Cat#R21001 |
| Neomycin | Thermo Fisher | Cat#21810031 |
| Polybrene | Merck Millipore | Cat#TR-1003-G |
| Lipofectamine 2000 | Invitrogen | Cat#11668030 |
| Dexamethasone | Sigma-Aldrich | Cat#D4902 |
| Vincristine | Selleckchem | Cat#S1241 |
| Doxorubicin | Sigma-Aldrich | Cat#D1515 |
| 6-Mercaptopurine | Abcam | Cat#ab142389 |
| Rapamycin | Selleckchem | Cat#S1039 |
| RAD001 | Selleckchem | Cat#S1120 |
| AZD2014 | Selleckchem | Cat#S2783 |
| Thapsigargin | Sigma-Aldrich | Cat#T9033 |
| BRD0703 | (Wagner et al., 2018) | N/A |
| BRD3731 | (Wagner et al., 2018) | N/A |
| RIPA buffer | Merck Millipore | Cat#20–188 |
| PhosSTOP phosphatase inhibitor | Roche | Cat#4906845001 |
| Laemmli sample buffer | Bio-Rad | Cat#161–0737 |
| b-mercaptoethanol | Sigma-Aldrich | Cat#M6250 |
| 4–12% bis-tris polyacrylamide gel | Thermo Fisher | Cat#NP0336 |
| PVDF membrane | Thermo Fisher | Cat# LC2005 |
| Azidohomoalanine (AHA) AlexaFluor488 | Thermo Fisher | Cat#C10289 |
| TAMRA alkyne | Thermo Fisher | Cat#T10183 |
| L-Asparagine | Sigma-Aldrich | Cat#A4159–25G |
| L-Glutamine | Sigma-Aldrich | Cat#G8540 |
| GelCode Blue Stain Reagent | Thermo Fisher | Cat#24590 |
| RPMI-1640 | Thermo Fisher | Cat#11875119 |
| Critical Commercial Assays | ||
| Caspase Glo 3/7 Assay | Promega | Cat#G8090 |
| DNeasy Blood and Tissue Kit | Qiagen | Cat#69504 |
| QIAquick PCR purification kit | Qiagen | Cat#28104 |
| Blood & Cell Culture DNA Maxi Kit | Qiagen | Cat#51194 |
| Z-VAD-FMK Caspase Inhibitor | Promega | Cat #G7231 |
| Deposited Data | ||
| Proteomics of CCRF-CEM cells treated with asparaginase and/or BRD0705 | ProteomeXchange | Dataset ID: PXD013061 |
| Experimental Models: Cell Lines | ||
| CCRF-CEM cells | ATCC | Cat#CCL-119 |
| Jurkat cells | ATCC | Cat#TIB-152 |
| PF382 cells | DSMZ | Cat#ACC-38 |
| DND41 cells | DSMZ | Cat# ACC-525 |
| MOLT4 cells | ATCC | Cat#CRL-1582 |
| OCI-AML2 cells | Alex Kentsis lab | N/A |
| SKM-1 cells | Alex Kentsis lab | N/A |
| THP-1 cells | Alex Kentsis lab | N/A |
| MHH CALL2 cells | DSMZ | Cat#ACC-341 |
| Normal human CD34+ hematopoietic progenitors | Fred Hutchinson Cancer Research Center | https://sharedresources.fredhutch.org/products/cd34-cells |
| Experimental Models: Organisms/Strains | ||
| NRG mice (NOD.Cg-Rag1<tm1Mom> Il2rg<tm1Wjl>/SzJ) | Jackson labs | 007799 |
| Oligonucleotides | ||
| See Table S5. | ||
| Recombinant DNA | ||
| LentiCas9-Blast | Addgene.org | Cat#52962 |
| LentiGuide-Puro | Addgene.org | Cat#52963 |
| Gecko v2 guide RNA library | Addgene.org | Cat#1000000048 and 1000000049 |
| shLuciferase | Broad RNAi Consortium | Cat#TRCN0000072243 |
| shNKD2 #1 | Broad RNAi Consortium | Cat#TRCN0000187580 |
| shNKD2 #3 | Broad RNAi Consortium | Cat#TRCN0000428381 |
| shLGR6 #2 | Broad RNAi Consortium | Cat#TRCN0000063619 |
| shLGR6 #4 | Broad RNAi Consortium | Cat#TRCN0000063621 |
| shAPC #1 | Broad RNAi Consortium | Cat#TRCN0000010296 |
| shAPC #2 | Broad RNAi Consortium | Cat#TRCN0000010297 |
| shGSK3α #1 | Broad RNAi Consortium | Cat#TRCN0000010340 |
| shGSK3α #4 | Broad RNAi Consortium | Cat#TRCN0000038682 |
| shGSK3β #2 | Broad RNAi Consortium | Cat#TRCN0000039564 |
| shGSK3β #6 | Broad RNAi Consortium | Cat#TRCN0000010551 |
| psPAX2 | Addgene.org | Cat#12260 |
| VSV.G | Addgene.org | Cat#14888 |
| Gag/pol | Addgene.org | Cat#14887 |
| pXPR_011 | Addgene.org | Cat#59702 |
| Constitutively active β-catenin (ΔN90) | Addgene.org | Cat#36985 |
| FBXW7 wild-type | Addgene.org | Cat#16652 |
| FBXW7 R465C mutant | Addgene.org | Cat#16653 |
| FBXW7 wild-type (CS-T3614-LX304–01-B) | Genecopoeia.com | |
| FBXW7 R465C mutant (EX-OL03574-LX304-B) | Genecopoeia.com | |
| 7TGC TOPFlash reporter | Addgene.org | Cat#24304 |
| Hyperactive ΔN-PSMA4 | This paper | N/A |
| GSK3α in pWZL expression vector | (Banerji et al., 2012) | N/A |
| Software and Algorithms | ||
| CrispRVariantsLite v1.1 | (Lindsay et al., 2016) | Version 1.1 |
| MAGeCK v.0.5.7 | (Li et al., 2014) | https://sourceforge.net/projects/mageck |
| CRISPOR | (Haeussler et al., 2016) | http://crispor.tefor.net/ |
| RStudio | v. 1.1.463 | http://www.rstudio.org |
| Other | ||