Table 1.

Enzymatic and nonenzymatic antioxidant systems in serum, haemolisates, and liver tissue of rats after WBC. The table presents descriptive statistics and results of two-way analysis of variance.

Temp.:	60		90		ANOVA
Time:	5	10	5	10	pTime	pTemp.	pInter.
Total SOD [NU/mL]	34.47 ± 0.30	34.33 ± 0.27	33.43 ± 0.32	32.95 ± 0.19	< 0.001	< 0.05	0.135
Total SOD [NU/mg Hgb]	23.73 ± 0.47	27.00 ± 0.15	31.50 ± 0.67	31.33 ± 0.91	< 0.001	< 0.001	< 0.001
Total SOD [NU/mg protein]	6.08 (6.05 – 6.09)	6.02 (6.01 – 6.05)	6.09 (6.08 – 6.10)	6.45 (6.30 – 6.60)	< 0.001	< 0.01	< 0.001
GPX [IU/g Hbg]	154.0 ( 154.0 – 157.0)	140.0 (139.0 – 141.0)	88.4 (88.3 – 88.6)	63.7 (63.5 – 93.9)	< 0.001	< 0.001	< 0.001
GPX [IU/mg protein]	9.93 ± 0.16	9.83 ± 0.08	5.95 ± 0.64	6.93 ± 0.32	< 0.001	< 0.05	< 0.01
CAT [IU/g Hbg]	403.0 ± 13.5	408.8 ± 9.2	539.2 ± 15.1	513.0 ± 8.6	< 0.001	< 0.05	< 0.01
CAT [IU/mg protein]	99.1 ± 0.5	96.2 ± 1.45	221.5 ± 6.2	235.0 ± 4.3	< 0.001	< 0.01	< 0.001
GST [IU/mg Hbg]	133.3 ± 3.3	113.5 ± 4.5	133.0 ± 1.4	124.7 ± 5.0	< 0.01	< 0.001	< 0.01
GST [IU/g protein]	140.2 ± 2.1	130.7 ± 3.3	147.7 ± 1.0	144.7 ± 0.8	< 0.001	< 0.001	< 0.001
GR [IU/g Hbg]	3.36 ± 0.20	3.48 ± 0.02	3.56 ± 0.02	3.69 ± 0.15	0.109	0.924	< 0.05
GR [IU/g protein]	92.00 ± 0.57	89.97 ± 1.05	93.15 ± 2.32	92.73 ± 1.23	< 0.001	< 0.05	0.184
MDA [mmol/L]	2.63 ( 2.62 – 2.65)	2.63 (2.61 – 2.65)	4.12 (4.01 – 4.14)	4.15 (4.13 – 4.65)	< 0.001	0.092	0.085
MDA [μmol/g Hgb]	319.5 ± 13.4	315.0 ± 23.6	349.0 ± 6.9	402.3 ± 24.4	< 0.001	< 0.01	< 0.01
MDA [μmol/g protein]	317.5 ± 12.5	339.2 ± 11.7	402.2 ± 6.7	315.3 ± 16.0	< 0.001	< 0.001	< 0.001
TAC [mmol/L]	3.40 ± 0.21	2.90 ± 0.05	1.67 ± 0.09	1.76 ± 0.12	< 0.001	< 0.001	< 0.001
pinter. – interaction between temperature and time

Statistical significance was set at p < 0.05. Group −60/5: 1 min of WBC stimulation, 5 days, −60°C; Group −60/10: 1 min of WBC stimulation, 10 days, −60°C; Group −90/5: 1 min of WBC stimulation, 5 days, −90°C; Group −90/10: 1 min of WBC stimulation, 10 days, −90°C.