Figure 4. ADIPOQ was involved in the miR-3666-mediated effects on the functions of pancreatic β-cells. A, INS-1 cells were transfected with pcDNA3.1 (vector) or pcDNA3.1-ADIPOQ (ADIPOQ), and the mRNA expression of ADIPOQ was determined by qRT-PCR. INS-1 cells were co-transfected with pcDNA3.1-ADIPOQ (or pcDNA3.1 vector) and miR-3666 mimic (or mimic-normal control (NC)). B, Glucose-stimulated insulin secretion was determined by ELISA assay. C, Cell proliferation was determined by MTT assay. D, Cell apoptosis was determined by flow cytometry. ^aP<0.05 compared to miR-3666 mimic NC+vector; ^bP<0.05 compared to miR-3666 mimic NC+(vector+ADIPOQ); ^cP<0.05 compared to miR-3666 mimic+vector. Data are reported as means±SE (n=3) and were compared with the t-test or ANOVA.