Figure 5. Western blot analysis of intracellular Ca²⁺-cycling proteins in myocardium from control (C, white bars) and obese (Ob, black bars) rats (n=6 in each group). A, Quantification of sarcoplasmic reticulum Ca²⁺-ATPase (SERCA2a), phospholamban (PLB), PLB serine-16 phosphorylation (pPLB Ser¹⁶), PLB threonine-17 phosphorylation (pPLB Thr¹⁷), ryanodine receptor (RYR), calsequestrin (CSQ), Na⁺/Ca²⁺ exchanger (NCX), and L-type Ca²⁺ channel. B, Representative bands of proteins evaluated by western blot. Data are reported as means±SD. Student's t-test for independent samples was used to compare data.