Figure 3.

Spatiotemporal expression of SCGB1A1, SCGB3A1, and SCGB3A2 during mouse lung gestation. (A) Immunohistochemistry for the expression of the three SCGBs in large and small airways at various mouse embryonic stages. Counterstained with light green. Scale bar: 50 µm for all panels. (B, C) qRT-PCR analysis for the expression of Scgb1a1, Scgb3a1, and Scgb3a2 mRNAs at various embryonic days through 3 weeks old. Relative expression plotted by logarithmic scale is shown in C. C57BL/6N mice were used for these analyses. A dot indicates an individual lung (N>5) for embryos/mice older than E16.5, and two combined lungs as one lung for embryos of E12.5 and 14.5. qRT-PCR was carried out in triplicate per sample. The average ± SE is shown. Abbreviations: SCGBs, Secretoglobins; NB, newborn.